It is difficult to know exactly when the shift from gelatine to agar occurred. As often happens for scientific breakthroughs, agar was likely adopted incrementally alongside the use of other growth media. In 1913, for example, the first diagnosis of Serratia marcescens as a human pathogen was made by growing it on agar as well as on potatoes.
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Number (9): Everything in this space must add up to 9. The answer is 6-5, placed horizontally; 1-4, placed vertically.